Construction of T cell hybridomas secreting allogeneic effect factor.
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چکیده
منابع مشابه
Construction of T cell hybridomas secreting allogeneic effect factor
T cell hybridoma lines were constructed by fusion of DBA/2 alloantigen-activated T cell blasts with the AKR thymoma line BW5147. Certain of the hybridomas prepared in this manner secreted spontaneously into their culture supernates biologically active molecules that displayed B cell- and T cell-activating properties characteristic of allogeneic effect factor (AEF). Cell surface phenotype analys...
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The development of human T cell hybridomas secreting immunoregulatory molecules has been an area of recent intense interest and investigation. Functional hybrids derived from both normal and malignant human T cells have been developed. These hybrids have been demonstrated to secrete interleukin 2 (IL-2) 1 (1), killer helper factor (2), and factors that help or suppress immunoglobulin production...
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Human T-T hybridomas were established by fusion of concanavalin A-activated OKT-4+ T cells with hypoxanthine guanine phosphoribosyl transferase-deficient as well as nondeficient T cell lines. Four hybrids were selected for further study. Supernatant from hybrid clone J1.3 specifically enhanced IgA production and secretion by isolated human B cells, with increases in IgA plaque-forming cells app...
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The success of long-term culture of normal human and murine B cells has been hampered by the limited availability of soluble factors capable of maintaining proliferation of activated B lymphocytes. Previous experiments using various culture-derived supernatants in a human system were unable to separate the activities of B cell growth factor (BCGF) and interleukin 2 (IL-2) by immunochemical mean...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1980
ISSN: 0022-1007,1540-9538
DOI: 10.1084/jem.152.4.956